We decided on 6 project goals to help steer our experiment.

Project Goals

1. Design a fast workflow for detection of miRNA.

2. Design a dog-bone strand with domains for miRNA attachment and molecular beacon attachment.

3. Synthesise the dog-bone probe, and circularize it, using an inexpensive method.

4. Get a standard curve for various RNA concentrations ranging from 1e-14 M to 1e-8 M.

5. Demonstrate system’s capability to select for the specific miRNA it was designed for.

6. Show our system is capable of producing quantitative results in human serum samples.